Replication of the colicin E1 plasmid in extracts of Escherichia coli: Uncoupling of leading strand from lagging strand synthesis

@article{Staudenbauer1979ReplicationOT,
  title={Replication of the colicin E1 plasmid in extracts of Escherichia coli: Uncoupling of leading strand from lagging strand synthesis},
  author={W. L. Staudenbauer and Eberhard Scherzinger and Erich Lanka},
  journal={Molecular and General Genetics MGG},
  year={1979},
  volume={177},
  pages={113-120}
}
The replication of the ColE1 plasmid was studied in extracts from E. coli dnaG mutants. It was found that the synthesis of the complementary strands of ColE1 DNA can be carried out in these extracts in two consecutive steps: (1) synthesis of the leading L strand independent of the dnaG function, and (2) synthesis of the lagging H strand depending upon addition of wild-type dnaG protein. In contrast to L strand synthesis, the latter reaction is insensitive to rifampicin and novobiocin. Both… CONTINUE READING