Replication of poliovirus RNA and subgenomic RNA transcripts in transfected cells.

@article{Collis1992ReplicationOP,
  title={Replication of poliovirus RNA and subgenomic RNA transcripts in transfected cells.},
  author={P S Collis and Brian O'Donnell and David J. Barton and John A. V. Rogers and James Bert Flanegan},
  journal={Journal of virology},
  year={1992},
  volume={66 11},
  pages={6480-8}
}
Full-length and subgenomic poliovirus RNAs were transcribed in vitro and transfected into HeLa cells to study viral RNA replication in vivo. RNAs with deletion mutations were analyzed for the ability to replicate in either the absence or the presence of helper RNA by using a cotransfection procedure and Northern (RNA) blot analysis. An advantage of this approach was that viral RNA replication and genetic complementation could be characterized without first isolating conditional-lethal mutants… CONTINUE READING