Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF

  title={Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF},
  author={Pascal Gamas and Albert Burger and Gordon G Churchward and Lucien G. Caro and David Galas and Michael Chandler},
  journal={Molecular and General Genetics MGG},
We have shown that the plasmid pSC101 is unable to be maintained in strains of E. coli carrying deletions in the genes himA and hip which specify the pleitropic heterodimeric DNA binding protein, IHF. We show that this effect is not due to a modulation of the expression of the pSC101 RepA protein, required for replication of the plasmid. Inspection of the DNA sequence of the essential replication region of pSC101 reveals the presence of a site, located between the DnaA binding-site and that of… CONTINUE READING

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A pSCl 01derived plasmid which shows no sequence homology to other commonly used cloning vectors

  • G Churchward, D Belin
  • 1984

Primary structure of the himA gene of E, coli: homology with DNA-binding protein HU and association with the phenylalanyl-tRNA synthetase operon

  • HI Miller
  • Cold Spring Harbor Symp Quant Biol
  • 1984
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