Replication of maize streak virus mutants in maize protoplasts: evidence for a movement protein.

@article{Boulton1993ReplicationOM,
  title={Replication of maize streak virus mutants in maize protoplasts: evidence for a movement protein.},
  author={Margaret I. Boulton and C. K. Pallaghy and M Chatani and Sandra Macfarlane and Jeffrey W. Davies},
  journal={Virology},
  year={1993},
  volume={192 1},
  pages={85-93}
}
We have used protoplasts derived from a maize (Black Mexican Sweet) suspension culture to study the replication of maize streak virus (MSV) wild-type (wt) and mutant DNA genomes. Following inoculation with plasmids containing multimeric copies of MSV, both single-stranded (ss) and double-stranded MSV DNA forms were produced, in proportions relative to those seen in infected plants. The immunodetection of coat protein (PV2) and geminate particles confirmed that the protoplasts were able to… CONTINUE READING

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