Replication of hepatitis C virus

  title={Replication of hepatitis C virus},
  author={Darius Moradpour and François Penin and Charles M. Rice},
  journal={Nature Reviews Microbiology},
Exciting progress has recently been made in understanding the replication of hepatitis C virus, a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma worldwide. The development of complete cell-culture systems should now enable the systematic dissection of the entire viral lifecycle, providing insights into the hitherto difficult-to-study early and late steps. These efforts have already translated into the identification of novel antiviral targets and the development… 
Novel hepatitis C drugs in current trials.
Unique ties between hepatitis C virus replication and intracellular lipids
Advances and challenges in studying hepatitis C virus in its native environment
Recent advances in culture and detection systems that facilitate the study of HCV in primary cells are discussed and use of these new models may help bridge the gap between in vitro studies and clinical research.
In vitro models for analysis of the hepatitis C virus life cycle
The present review focuses on the progress made towards the establishment of suitable in vitro and in vivo models for the analysis of HCV infection and the development of both protective and therapeutic strategies againstHCV infection.
Experimental models for hepatitis C viral infection
An overview of current models of hepatitis C virus infection is given, including in vitro cell culture systems and in vivo small‐animal models, to discuss their limitations, and provide future perspectives for research directed at the prevention and cure of HCV.
The molecular and structural basis of advanced antiviral therapy for hepatitis C virus infection
Insight into the structures of these proteins and the mechanisms by which they contribute to the HCV replication cycle are reviewed, and how these insights have facilitated the development of new, directly acting antiviral compounds that have started to enter the clinic are discussed.
Hepatitis C Virus
The establishment of a system for the study of the complete virus lifecycle in tissue culture has led to many major advances and provides opportunities for further therapeutic developments, which are described in this article.
Hepatitis C virus vaccine development: old challenges and new opportunities
Recent progresses on HCV vaccine development are summarized and the views on the rationale and strategy to develop an effective HCV Vaccine are presented.


Unravelling hepatitis C virus replication from genome to function
Since the discovery of the hepatitis C virus over 15 years ago, scientists have raced to develop diagnostics, study the virus and find new therapies. Yet virtually every attempt to dissect this
Challenges and successes in developing new therapies for hepatitis C
Preclinical evidence is accumulating that development of resistance will eventually limit the efficacy of new drugs, and combinations of multiple agents will be required to treat chronic HCV infection.
Robust hepatitis C virus infection in vitro.
A simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture is reported.
Structural biology of hepatitis C virus
Structural analyses of HCV components provide an essential framework for understanding of the molecular mechanisms ofHCV polyprotein processing, RNA replication, and virion assembly and may contribute to a betterUnderstanding of the pathogenesis of hepatitis C.
Complete Replication of Hepatitis C Virus in Cell Culture
A full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc) is described, suggesting that this in vitro system will aid in the search for improved antiviral compounds.
Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA.
This work defines the structure of a functional HCV genome RNA and proves that HCV alone is sufficient to cause disease.
Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.
This work defines the structure of HCV replicons functional in cell culture and provides the basis for a long-sought cellular system that should allow detailed molecular studies ofHCV and the development of antiviral drugs.
Efficient initiation of HCV RNA replication in cell culture.
This work establishes a robust, cell-based system for genetic and functional analyses of HCV replication and identifies multiple independent adaptive mutations that cluster in the HCV nonstructural protein NS5A and confer increased replicative ability in vitro.
Characterization of long-term cultures of hepatitis C virus
The human T- and B-cell lines HPBMa10-2 and Daudi produced infectious hepatitis C virus for more than 1 year after infection and one particular HCV sequence which was present in minor quantities in the inoculum persisted.