Replication of derivatives of the broad host range plasmid RK2 in two distantly related bacteria.

@article{Schmidhauser1983ReplicationOD,
  title={Replication of derivatives of the broad host range plasmid RK2 in two distantly related bacteria.},
  author={Thomas J Schmidhauser and M. Filutowicz and D. R. Helinski},
  journal={Plasmid},
  year={1983},
  volume={9 3},
  pages={325-30}
}
A 0.7-kb segment of the broad host range plasmid RK2 containing the replication origin of this plasmid will replicate in Escherichia coli and Pseudomonas putida when this segment is joined to a 1.8-kb region of RK2 designated traA*. The presence of another region of RK2, designated trfB, that previously was implicated in RK2 replication had no effect on the maintenance of the RK2 trfA*-oriV replicon in these two organisms. These observations indicate a requirement for a minimal account of… CONTINUE READING

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