Replication of adenovirus DNA in vitro is ATP-independent.

@article{Pronk1994ReplicationOA,
  title={Replication of adenovirus DNA in vitro is ATP-independent.},
  author={Rob Pronk and W. D. van Driel and Peter C. van der Vliet},
  journal={FEBS letters},
  year={1994},
  volume={337 1},
  pages={
          33-8
        }
}
Using a reconstituted system for adenovirus DNA replication we tested the requirements for ATP and divalent cations. At the standard Mg2+ concentration ATP stimulated initiation 5 to 10-fold. However, this effect was caused by complexing Mg2+. At the optimal Mg2+ concentration ATP was not required for initiation or elongation. Besides Mg2+ also Mn2+, Ca2+ and Ba2+ were shown to support initiation whereas for elongation only Mg2+ was accepted. Since Mn2+ could efficiently be used for DNA chain… CONTINUE READING
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