Replication of 2-hydroxyadenine-containing DNA and recognition by human MutSalpha.

  title={Replication of 2-hydroxyadenine-containing DNA and recognition by human MutSalpha.},
  author={Flavia Barone and Scott D. McCulloch and Peter Macpherson and Giovanni Maga and Masami Yamada and Takehiko Nohmi and Anna Minoprio and Filomena Mazzei and T. A. Kunkel and P. Karran and Margherita Bignami},
  journal={DNA repair},
  volume={6 3},
2-Hydroxyadenine (2-OH-A), a product of DNA oxidation, is a potential source of mutations. We investigated how representative DNA polymerases from the A, B and Y families dealt with 2-OH-A in primer extension experiments. A template 2-OH-A reduced the rate of incorporation by DNA polymerase alpha (Pol alpha) and Klenow fragment (Kf(exo-)). Two Y family DNA polymerases, human polymerase eta (Pol eta) and the archeal Dpo4 polymerase were affected differently. Bypass by Pol eta was very… CONTINUE READING

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