Replication genes of plasmid pE194-cop and repF: transcripts and encoded proteins.

@article{Byeon1990ReplicationGO,
  title={Replication genes of plasmid pE194-cop and repF: transcripts and encoded proteins.},
  author={W H Byeon and Bernard Weisblum},
  journal={Journal of bacteriology},
  year={1990},
  volume={172 10},
  pages={5892-900}
}
In vivo transcription of the replication region of plasmid pE194 yeidls two classes of mRNAs that encode Cop and RepF proteins, respectively. These transcripts are oriented 5' to 3' exclusively in the clockwise direction on the standard map. The cop region contains an open reading frame capable of encoding a 55-amino-acid protein that was demonstrated electrophoretically as a 6-kilodalton product synthesized in Bacillus subtilis minicells and chemically by N-terminal sequencing of a 116… CONTINUE READING