Replication and packaging of transmissible gastroenteritis coronavirus-derived synthetic minigenomes.

@article{Izeta1999ReplicationAP,
  title={Replication and packaging of transmissible gastroenteritis coronavirus-derived synthetic minigenomes.},
  author={Ander Izeta and Cristian Smerdou and Sara Alonso and Zoltan P{\'e}nzes and Ana M M{\'e}ndez and Juan Plana-Dur{\'a}n and Luis Enjuanes},
  journal={Journal of virology},
  year={1999},
  volume={73 2},
  pages={1535-45}
}
The sequences involved in the replication and packaging of transmissible gastroenteritis virus (TGEV) RNA have been studied. The structure of a TGEV defective interfering RNA of 9.7 kb (DI-C) was described previously (A. Mendez, C. Smerdou, A. Izeta, F. Gebauer, and L. Enjuanes, Virology 217: 495-507, 1996), and a cDNA with the information to encode DI-C RNA was cloned under the control of the T7 promoter. The molecularly cloned DI-C RNA was replicated in trans upon transfection of helper virus… CONTINUE READING