Replacing immunoassays with tryptic digestion-peptide immunoaffinity enrichment and LC-MS/MS.

For decades, immunoassays have provided the framework for protein biomarker studies in clinical medicine and in therapeutic monitoring for drug development. At the same time, investigators have uncovered many issues that make immunoassays unreliable in many human serum and plasma samples. LC-MS/MS after tryptic digestion of proteins is potentially an… CONTINUE READING