Replacement of chromosome segments with altered DNA sequences constructed in vitro.

@article{Scherer1979ReplacementOC,
  title={Replacement of chromosome segments with altered DNA sequences constructed in vitro.},
  author={Stewart Scherer and Ronald W. Davis},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1979},
  volume={76 10},
  pages={4951-5}
}
We have developed a method that may be of general application for the stable introduction of foreign sequences or deletions, constructed in vitro, into the chromosomes of Saccharomyces cerevisiae. No vector sequences are present in the final strains. Ability to transform cells with DNA, availability of a single selective marker, and integration of the… CONTINUE READING