Replacement of Thymidylic Acid by Deoxyuridylic Acid in the Deoxyribonucleic Acid of a Transducing Phage for Bacillus subtilis

  title={Replacement of Thymidylic Acid by Deoxyuridylic Acid in the Deoxyribonucleic Acid of a Transducing Phage for Bacillus subtilis},
  author={I. Takahashi and Julius Marmur},
THE findings that the thermal denaturation temperature (Tm) of DNA from a wide variety of bacterial, plant and animal sources as well as the buoyant density in a cæsium chloride gradient are linearly related to the guanine plus cytosine (G plus C) base composition1–2 have made it possible to search for the existence of DNA samples which would show an anomalous behaviour with respect to these two parameters. Native DNA preparations which show no correlation between their buoyant density and Tm… 

2-Aminoadenine is an adenine substituting for a base in S-2L cyanophage DNA

The isolation and identification of the base 2-aminoadenine and its deoxyribonucleoside from S-2L cyanophage DNA is reported and some unusual properties of this DNA in which adenine is completely substituted with 2-AMB are described.

DNase Specific for Uracil-Containing Bacteriophage DNA

A DNase from Bacillus subtilis which specifically hydrolyzes native DNA of phage PBS 1 has been purified and characterized and yielded deoxyuridine and oliogonucleotides of various sizes.

DNA glycosylases, endonucleases for apurinic/apyrimidinic sites, and base excision-repair.

  • T. Lindahl
  • Biology
    Progress in nucleic acid research and molecular biology
  • 1979

New Deoxyribonucleic Acid Polymerase Induced by Bacillus subtilis Bacteriophage PBS2

The deoxyribonucleic acid of Bacillus subtilis phage PBS2 has been confirmed to contain uracil instead of thymine, and this phage-induced polymerase may be responsible for the synthesis of Uracil-containing DNA during PBS2 phage infection.

Synthesis of the Unusual DNA of Bacillus subtilis Bacteriophage SP-15

Analysis of the bases from hydrolyzed DNA of labeled phage or infected cells indicated that the uracil was incorporated into the DNA as such (presumably via deoxyuridine triphosphate) and later converted to DHPU and thymine at the macromolecular level.

Modified polynucleotides. V. Slow-down of nuclease action by 5-alkyluracil-containing DNAs.

  • J. SagiL. Otvös
  • Biology, Chemistry
    Biochemical and biophysical research communications
  • 1980

Hypermodified bases in DNA

  • J. H.Gommers‐AmptP. Borst
  • Biology, Chemistry
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1995
This review gives an overview of the modified DNA bases identified thus far, with emphasis on the “very unusual” or hypermodified DNA bases.