Repetitive shuttling of a motor protein on DNA

@article{Myong2005RepetitiveSO,
  title={Repetitive shuttling of a motor protein on DNA},
  author={Sua Myong and Ivan Rasnik and Chirlmin Joo and Timothy M. Lohman and Taekjip Ha},
  journal={Nature},
  year={2005},
  volume={437},
  pages={1321-1325}
}
Many helicases modulate recombination, an essential process that needs to be tightly controlled. Mutations in some human disease helicases cause increased recombination, genome instability and cancer. To elucidate the potential mode of action of these enzymes, here we developed a single-molecule fluorescence assay that can visualize DNA binding and translocation of Escherichia coli Rep, a superfamily 1 DNA helicase homologous to Saccharomyces cerevisiae Srs2. Individual Rep monomers were… CONTINUE READING

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Auto-inhibition of E. coli Rep monomer helicase activity by its 2B sub-domain

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