Repetitive Somatic Embryogenesis of Ocotea catharinensis Mez. (Lauraceae): Effect of Somatic Embryo Developmental Stage and Dehydration

@article{Catarina2004RepetitiveSE,
  title={Repetitive Somatic Embryogenesis of Ocotea catharinensis Mez. (Lauraceae): Effect of Somatic Embryo Developmental Stage and Dehydration},
  author={Claudete Santa Catarina and Alessandra dos Santos Olmedo and Geraldine de Andrade Meyer and Jonice Macedo and Wagner de Amorim and Ana Maria Viana},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={78},
  pages={55-62}
}
Repetitive embryogenesis of Ocotea catharinensis from globular/early cotyledonary somatic embryos was successfully supported by WPM supplemented with 22.7 g l−1 sorbitol, 20 g l−1 sucrose, 400 mg l−1 glutamine and 2 g l−1 Phytagel. The best medium to induce repetitive embryogenesis in cotyledonary somatic embryos was half strength WPM supplemented with 20 g l−1 sucrose, 400 mg l−1 glutamine, 1.5 g l−1 activated charcoal and 2 g l−1 Phytagel. The mature somatic embryos gradually air dehydrated… CONTINUE READING
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