Repeated use of surrogate mothers for embryo transfer in the mouse.

@article{Kolbe2012RepeatedUO,
  title={Repeated use of surrogate mothers for embryo transfer in the mouse.},
  author={Thomas Kolbe and Rupert Palme and Chadi Touma and Thomas R{\"u}licke},
  journal={Biology of reproduction},
  year={2012},
  volume={86 1},
  pages={1-6}
}
Embryo transfer in mice is a crucial technique for generation of transgenic animals, rederivation of contaminated lines, and revitalization of cryopreserved strains, and it is a key component of assisted reproduction techniques. It is common practice to use females only once as surrogate mothers. However, their reuse for a second embryo transfer could provide hygienic and economic advantages and conform to the concept of the 3Rs (replace, reduce, refine). This investigation evaluated the… CONTINUE READING