Repeated application of first-layer antiserum improves immunofluorescence staining: a modification of the indirect immunofluorescence staining procedure

Abstract

Repeated application of the first-layer antiserum in the indirect immunofluorescence technique considerably improves the immunostaining. The modified method reveals more antigenic sites and increases the contrast between specific and background stainings, particularly where sparsely distributed antigenic areas are to be investigated. The effect of this… (More)
DOI: 10.1007/BF01002701

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@article{Gu1983RepeatedAO, title={Repeated application of first-layer antiserum improves immunofluorescence staining: a modification of the indirect immunofluorescence staining procedure}, author={J. Gu and Kazi Islam and Justyna Polak}, journal={The Histochemical Journal}, year={1983}, volume={15}, pages={475-482} }