Repair of 8-methoxypsoralen photoinduced cross-links in yeast

  title={Repair of 8-methoxypsoralen photoinduced cross-links in yeast},
  author={Enrico Cundari and M. Dardalhon and S Brovedani Rousset and Dietrich Averbeck},
  journal={Molecular and General Genetics MGG},
The repair of interstrand cross-links induced by 8-methoxypsoralen plus UVA (365 nm) radiation DNA was analyzed in diploid strains of the yeast Saccharomyces cerevisiae. The strains employed were the wild-type D7 and derivatives homozygous for the rad18-1 or the rad3-12 mutation. Alkaline step-elution and electron microscopy were performed to follow the process of induction and removal of photoinduced crosslinks. In accordance with previous reports, the D7 rad3-12 strain failed to remove the… CONTINUE READING