Reovirus Protein s NS Binds in Multiple Copies to Single-Stranded RNA and Shares Properties with Single-Stranded DNA Binding Proteins

@inproceedings{Gillian2000ReovirusPS,
  title={Reovirus Protein s NS Binds in Multiple Copies to Single-Stranded RNA and Shares Properties with Single-Stranded DNA Binding Proteins},
  author={Anne Lynn Gillian and Stephen C. Schmechel and Jonathan Livny and Leslie A. Schiff and Max L Nibert},
  year={2000}
}
Reovirus nonstructural protein sNS interacts with reovirus plus-strand RNAs in infected cells, but little is known about the nature of those interactions or their roles in viral replication. In this study, a recombinant form of sNS was analyzed for in vitro binding to nucleic acids using gel mobility shift assays. Multiple units of sNS bound to single-stranded RNA molecules with positive cooperativity and with each unit covering about 25 nucleotides at saturation. The sNS protein did not bind… CONTINUE READING

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