Reorganization of Microtubules During Mitosis in Dictyostelium : Dissociation From MTOC and Selective Assembly / Disassembly In Situ

@inproceedings{KitanishiYumura2004ReorganizationOM,
  title={Reorganization of Microtubules During Mitosis in Dictyostelium : Dissociation From MTOC and Selective Assembly / Disassembly In Situ},
  author={Toshiko Kitanishi-Yumura and Yoshio Fukui},
  year={2004}
}
We applied the “agar-overlay ” immunofluorescence technique (Yumura, S., H. Mori, and Y. Fukui. J . Cell Biol. 99:894-899, 1984) to a semisynchronous culture of Dictyostelium discoideum for studying the organization changes in the microtubule system during mitosis. Using a fluorescent DNA dye DAPI (4‘,6’ diamidino-2-phenylindole), chromatin fibers and individual chromosomes were visible in cells prepared by this method, whereby the mitotic phase could be critically evaluated. We found that a… CONTINUE READING