Renouncing electroneutrality is not free of charge: switching on electrogenicity in a Na+-coupled phosphate cotransporter.

@article{Bacconi2005RenouncingEI,
  title={Renouncing electroneutrality is not free of charge: switching on electrogenicity in a Na+-coupled phosphate cotransporter.},
  author={Andrea Bacconi and Leila V. Virkki and Juerg Biber and Heini Murer and Ian Cameron Forster},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 35},
  pages={12606-11}
}
Renal type IIa Na+-coupled inorganic phosphate (Pi) cotransporters (NaPi-IIa) mediate divalent Pi transport in an electrogenic manner, whereas the renal type IIc isoform (NaPi-IIc) is electroneutral, yet it shows high sequence identity with NaPi-IIa. Dual uptake (32Pi/22Na) assays confirmed that NaPi-IIc displayed Na+-coupled Pi cotransport with a 2:1 (Na+:Pi) stoichiometry compared with 3:1 established for NaPi-IIa. This finding suggested that the electrogenicity of NaPi-IIa arises from the… CONTINUE READING