Renaturation with simultaneous purification of rhG-CSF from Escherichia coli by ion exchange chromatography.

Abstract

Protein refolding is a key step for the production of recombinant proteins, especially at large scales, and usually their yields are very low. Application of liquid chromatography to protein refolding is an exciting step forward for this field. In this work, recombinant human granulocyte colony-stimulating factor (rhG-CSF) expressed in Escherichia coli was… (More)

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@article{Wang2007RenaturationWS, title={Renaturation with simultaneous purification of rhG-CSF from Escherichia coli by ion exchange chromatography.}, author={Chaozhan Wang and Lili Wang and Xindu Geng}, journal={Biomedical chromatography : BMC}, year={2007}, volume={21 12}, pages={1291-6} }