Renaturation, purification and characterization of streptokinase expressed as inclusion body in recombinant E. coli.

@article{Babu2008RenaturationPA,
  title={Renaturation, purification and characterization of streptokinase expressed as inclusion body in recombinant E. coli.},
  author={P V Cherish Babu and Venkatesh Srinivas and VS Krishna Mohan and E. Krishna},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2008},
  volume={861 2},
  pages={218-26}
}
Recombinant protein purification is facilitated using high expression systems which produce larger quantities of streptokinase protein as inclusion bodies. As the accumulation of active streptokinase is toxic to the host cells, we have optimized the conditions to achieve large amounts of streptokinase in the form of inclusion bodies. The solubility and yield of pure protein are highly dependent on various combinations of chemical additives, ionic and non-ionic detergents and salts, with… CONTINUE READING