Renal sodium-D-glucose cotransport system. Involvement of tyrosine residues in sodium-transporter interaction.

Abstract

Using brush-border membrane vesicles isolated from calf kidney cortex the effect of tyrosine-reactive reagents on sodium-dependent D-glucose transport was investigated. Treatment of the membranes for 60 min with NBD-Cl (7-chloro-4-nitrobenzo-2-oxa-1,3-diazole), N-acetylimidazole or tetranitromethane decreased D-glucose uptake 50, 70 and 40%, respectively. Tracer exchange experiments revealed that the inhibition of transport is due to a direct modification of the sodium-D-glucose cotransport system. The modification by NBD-Cl decreases the apparent Vmax of the transport system with respect to its interaction with sodium. In addition, the rate of inactivation of the transport system by NBD-Cl is reduced in the presence of high concentrations of sodium. The results indicate that tyrosine residues play an essential role in sodium-D-glucose cotransport and are probably involved in the binding and/or transport of sodium by the sodium-D-glucose cotransport system.

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@article{Lin1982RenalSC, title={Renal sodium-D-glucose cotransport system. Involvement of tyrosine residues in sodium-transporter interaction.}, author={J. T. Lin and A Stroh and R . Kinne}, journal={Biochimica et biophysica acta}, year={1982}, volume={692 2}, pages={210-7} }