Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption.

@article{Abdullah2005RemovalOP,
  title={Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption.},
  author={Norhani Abdullah and Howard A. Chase},
  journal={Biotechnology and bioengineering},
  year={2005},
  volume={92 4},
  pages={501-13}
}
Enzymatic methods have been used to cleave the C- or N-terminus polyhistidine tags from histidine tagged proteins following expanded bed purification using immobilized metal affinity chromatography (IMAC). This study assesses the use of Factor Xa and a genetically engineered exopeptidase dipeptidyl aminopeptidase-1 (DAPase-1) for the removal of C-terminus… CONTINUE READING