Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages.

@article{Singer1989RemovalOF,
  title={Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages.},
  author={Kay Hiemstra Singer and Richard M Scearce and D T Tuck and Leona P. Whichard and Stephen Denning and Barton F Haynes},
  journal={The Journal of investigative dermatology},
  year={1989},
  volume={92 2},
  pages={166-70}
}
A complement fixing IgM monoclonal antibody (1B10) that reacts with surface membrane molecules of human fibroblasts, tissue macrophages, and peripheral monocytes was produced. In Western blot analysis of detergent extracts of cultured human foreskin fibroblasts, antibody 1B10 detected protein bands of Mr 43,000 and 72-80,000. We used the 1B10 antibody with complement to eliminate most 1B10 positive nonepithelial cells from thymic epithelial (TE) cell cultures, thereby allowing us to grow highly… CONTINUE READING

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