Removal of endotoxins from plasmid DNA: analysis of aggregative interaction of mobile divalent metal cations with endotoxins and plasmid DNA.

@article{Ongkudon2012RemovalOE,
  title={Removal of endotoxins from plasmid DNA: analysis of aggregative interaction of mobile divalent metal cations with endotoxins and plasmid DNA.},
  author={Clarence M. Ongkudon and Emma K Hodges and Kathleen E Murphy and Michael K Danquah},
  journal={Journal of separation science},
  year={2012},
  volume={35 22},
  pages={3208-16}
}
Endotoxin lipopolysaccharide removal from plasmid DNA-based vaccine remains a very challenging task for bioprocess engineers. This paper examined the potential use and advantages of divalent cation (Zn(2+), Ca(2+), Mg(2+)) induced aggregation as a plasmid DNA purification method for lipopolysaccharide removal. Analysis of zeta potential, hydrodynamic size, percentage of aggregation; UV-Vis spectroscopy and electron microscopy were performed to determine the optimal cation for preferential… CONTINUE READING

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