• Biology, Medicine
• Published in
  Molecular therapy : the…
  2006
• DOI:10.1016/j.ymthe.2005.11.024

Removal of empty capsids from type 1 adeno-associated virus vector stocks by anion-exchange chromatography potentiates transgene expression.

@article{Urabe2006RemovalOE,
  title={Removal of empty capsids from type 1 adeno-associated virus vector stocks by anion-exchange chromatography potentiates transgene expression.},
  author={Masashi Urabe and Ke-Qin Xin and Yoko Obara and Takayo Nakakura and Hiroaki Mizukami and Akihiro Kume and Kenji Okuda and Keiya Ozawa},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2006},
  volume={13 4},
  pages={
          823-8
        }
}

• Masashi Urabe, Ke-Qin Xin, +5 authors Keiya Ozawa

Production of recombinant adeno-associated virus (rAAV) results in substantial quantities of empty capsids or virus-like particles (VLPs), virus protein shells without the vector genome. The contaminating VLPs would interfere with transduction by competing for cell-surface receptors and, when administered in vivo, contribute to antigen load, which may elicit a stronger immune response. Density-gradient ultracentrifugation provides a means to separate VLPs from rAAV particles, but is not… CONTINUE READING

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