Removal of empty capsids from type 1 adeno-associated virus vector stocks by anion-exchange chromatography potentiates transgene expression.

@article{Urabe2006RemovalOE,
  title={Removal of empty capsids from type 1 adeno-associated virus vector stocks by anion-exchange chromatography potentiates transgene expression.},
  author={Masashi Urabe and Ke-Qin Xin and Yoko Obara and Takayo Nakakura and Hiroaki Mizukami and Akihiro Kume and Kenji Okuda and Keiya Ozawa},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2006},
  volume={13 4},
  pages={
          823-8
        }
}
Production of recombinant adeno-associated virus (rAAV) results in substantial quantities of empty capsids or virus-like particles (VLPs), virus protein shells without the vector genome. The contaminating VLPs would interfere with transduction by competing for cell-surface receptors and, when administered in vivo, contribute to antigen load, which may elicit a stronger immune response. Density-gradient ultracentrifugation provides a means to separate VLPs from rAAV particles, but is not… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 23 CITATIONS

Viral Vectors for Gene Therapy

  • Methods in Molecular Biology
  • 2011
VIEW 7 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED

Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells

  • Molecular therapy. Methods & clinical development
  • 2019
VIEW 1 EXCERPT
CITES METHODS