Removal of background label in immunocytochemistry with the apolar Lowicryls by using washed protein A-gold-precoupled antibodies in a one-step procedure.

Abstract

Antiserum mixed with protein A-gold produces aggregates that can be washed by centrifugation and redissociated by ultrasonication. These precoupled antibodies are used for immunocytochemical labeling in a one-step procedure and reveal a very low background on sections of material embedded in polar and apolar Lowicryls. Controls have shown that the… (More)

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