Remote detection of buried landmines using a bacterial sensor

  title={Remote detection of buried landmines using a bacterial sensor},
  author={Shimshon Belkin and Sharon Yagur‐Kroll and Yossef Kabessa and Victor Korouma and Tali Septon and Yonatan Anati and Cheinat Zohar-Perez and Zahi Rabinovitz and Amos Nussinovitch and Aharon J. Agranat},
  journal={Nature Biotechnology},
VOLUME 35 NUMBER 4 APRIL 2017 NATURE BIOTECHNOLOGY with a novel mode of action is approved for marketing? We submit that three decades of soliciting public engagement has not improved the public acceptance cited by advocates as a justification for the efforts, and that subordinating evidence-based policy making to emotional or political calculations has neither increased public acceptance nor encouraged innovation. We would prefer to heed the caveat of Barbara Keating-Edh, who testified before… 

Detection of buried explosives with immobilized bacterial bioreporters

This is the first report demonstrating the detection of a buried landmine with a luminescent microbial bioreporter, designed to bioluminescence in response to minute concentrations of either TNT or 2,4‐dinitotoluene (DNT)‐based mines.

Bacterial bioreporters for the detection of trace explosives: performance enhancement by DNA shuffling and random mutagenesis

Improved DNT-detection capabilities of microbial sensor strains for buried explosives was molecularly enhanced, and the re-engineered constructs exhibited superior detection of trace explosives.

Enhancing DNT Detection by a Bacterial Bioreporter: Directed Evolution of the Transcriptional Activator YhaJ

The directed evolution of YhaJ, the transcriptional activator of the yqjF gene promoter, the sensing element of the bioreporter’s molecular circuit, resulted in a 37-fold reduction of the detection threshold, as well as significant enhancements to signal intensity and response time, rendering this sensor strain more suitable for detecting the minute concentrations of DNT in the soil above buried landmines.

A primer on emerging field-deployable synthetic biology tools for global water quality monitoring

Current water quality monitoring strategies enabled by synthetic biology are described and compare them to previous approaches used to detect three priority water contaminants, as well as explaining the potential for engineered biosensors to simplify and decentralize waterquality monitoring.

Biosensors for explosives: State of art and future trends

Unlocking the Potential of SynBio for Improving Livelihoods in Africa

Synthetic biology (SynBio) is an interdisciplinary field that has developed rapidly in the last two decades. It involves the design and construction of new biological systems and processes from

Quantitative chemical sensing employing bioluminescent bacteria

This methodology to include quantitative sensing of the contents of a target material (TM) in a given sample by exploiting the dependence of the bioluminescence produced by the bacteria on the content of the TM in the inspected sample is extended.

Cell-free biosensors for rapid detection of water contaminants

A cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water, and it is shown that adding RNA circuitry can invert responses, reduce crosstalk and improve sensitivity without protein engineering.

Bioluminescent bacterial biosensor for large-scale field deployment

A biosensing module in which live bacteria, genetically “tailored” to respond to the presence of a specific target material, constitute the core sensing element, reporting their response by bioluminescence, was developed for the detection of buried landmines.



Alternatives for landmine detection

Abstract : Antipersonnel mines remain a significant international threat to civilians despite recent intense efforts by the United States, other developed countries, and humanitarian aid

Escherichia coli bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene

Performance of the yqjF-based sensor was significantly improved in terms of detection threshold, response time, and signal intensity, following two rounds of random mutagenesis in the promoter region, and it was further demonstrated that both 2,4,6-TNT and 2, 4-DNT are metabolized by E. coli and that the actual induction of both yqJF and ybiJ is caused by yet unidentified degradation products.

Composition of vapors evolved from military TNT as influenced by temperature, solid composition, age and source

Abstract : A number of domestic and foreign military TNT samples were analyzed by a gas chromatographic headspace technique. The method allowed the determination of the vapor pressure of TNT and the

Polymer Macro- and Micro-Gel Beads: Fundamentals and Applications

Physical Properties of Beads and Their Estimation.- Bead Formation, Strengthening, and Modification.- Methods and Mathematical Models for the Drying of Polymeric Beads.- Food and Biotechnological

Detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene by an Escherichia coli bioreporter: performance enhancement by directed evolution

A directed evolution process to enhance the performance of a previously described E. coli-based bioreporter harboring a plasmid-borne genetic fusion between the yqjF gene promoter and either luxCDABE or gfp genes is reported, yielding a fourth-generation sensor that displayed significantly improved 2,4-DNT detection characteristics compared to the wild-type and to previous generations.

Analysis of explosives-related signature chemicals in soil samples collected near buried landmines

  • Technical Report No. ERDC TR-00-5. (US Army Corps of Engineers,
  • 2000