Remodeling of the Rad51 DNA strand-exchange protein by the Srs2 helicase.

@article{Sasanuma2013RemodelingOT,
  title={Remodeling of the Rad51 DNA strand-exchange protein by the Srs2 helicase.},
  author={Hiroyuki Sasanuma and Yuko Furihata and Miki Shinohara and Akira Shinohara},
  journal={Genetics},
  year={2013},
  volume={194 4},
  pages={859-72}
}
Homologous recombination is associated with the dynamic assembly and disassembly of DNA-protein complexes. Assembly of a nucleoprotein filament comprising ssDNA and the RecA homolog, Rad51, is a key step required for homology search during recombination. The budding yeast Srs2 DNA translocase is known to dismantle Rad51 filament in vitro. However, there is limited evidence to support the dismantling activity of Srs2 in vivo. Here, we show that Srs2 indeed disrupts Rad51-containing complexes… CONTINUE READING

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