Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers

@inproceedings{Kolacsek2010ReliableTM,
  title={Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers},
  author={Orsolya Kolacsek and Vir{\'a}g Kr{\'i}zsik and Anita Schamberger and Zsuzsa Erdei and {\'A}gota Ap{\'a}ti and Gy{\"o}rgy V{\'a}rady and Lajos M{\'a}t{\'e}s and Zsuzsanna Izsv{\'a}k and Zolt{\'a}n Ivics and Bal{\'a}zs Sarkadi and Tam{\'a}s I. Orb{\'a}n},
  booktitle={Mobile DNA},
  year={2010}
}
The transposon-based gene delivery technique is emerging as a method of choice for gene therapy. The Sleeping Beauty (SB) system has become one of the most favored methods, because of its efficiency and its random integration profile. Copy-number determination of the delivered transgene is a crucial task, but a universal method for measuring this is lacking. In this paper, we show that a real-time quantitative PCR-based, transgene-independent (qPCR-TI) method is able to determine SB transposon… CONTINUE READING
13 Citations
29 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 29 references

Similar Papers

Loading similar papers…