Reliable quantification of hematopoietic chimerism after allogeneic transplantation for acute leukemia using amplification by real-time PCR of null alleles and insertion/deletion polymorphisms

@article{JimnezVelasco2005ReliableQO,
  title={Reliable quantification of hematopoietic chimerism after allogeneic transplantation for acute leukemia using amplification by real-time PCR of null alleles and insertion/deletion polymorphisms},
  author={Antonio Jim{\'e}nez-Velasco and Manuel Barrios and Jos{\'e} Rom{\'a}n-G{\'o}mez and Germ{\'a}n Navarro and Ismael Bu{\~n}o and Juan Antonio Castillejo and Ana Isabel Rodr{\'i}guez and Gracia Garc{\'i}a-Gemar and Antonio Torres and Ana Isabel Heiniger},
  journal={Leukemia},
  year={2005},
  volume={19},
  pages={336-343}
}
Increasing mixed chimerism (MC) after allogeneic stem cell transplantation (SCT) has been associated with a high risk of relapse in acute leukemia. We evaluated a new method for chimerism detection, based on the quantitative real-time PCR (qrt-PCR) amplification of null alleles or insertion/deletion polymorphisms (indels). All qrt-PCR assays with null alleles and indels attained a sensitivity of at least 10−4, as well as good intra- and interassay concordance, and a high accuracy in experiments… CONTINUE READING

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Long - term follow - up of persisting mixed chimerism after partially T celldepleted allogeneic

MF Bertheas, M Lafage, +3 authors P Viens
stem cell transplantation Leukemia • 2002

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