Reliable gene expression analysis by reverse transcription-quantitative PCR: reporting and minimizing the uncertainty in data accuracy.

@article{Remans2014ReliableGE,
  title={Reliable gene expression analysis by reverse transcription-quantitative PCR: reporting and minimizing the uncertainty in data accuracy.},
  author={Tony Remans and E. E. Keunen and Geert Jan Bex and Karen H. G. Smeets and Jaco Vangronsveld and Ann Cuypers},
  journal={The Plant cell},
  year={2014},
  volume={26 10},
  pages={3829-37}
}
Reverse transcription-quantitative PCR (RT-qPCR) has been widely adopted to measure differences in mRNA levels; however, biological and technical variation strongly affects the accuracy of the reported differences. RT-qPCR specialists have warned that, unless researchers minimize this variability, they may report inaccurate differences and draw incorrect biological conclusions. The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines describe… CONTINUE READING
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