Reliable determination of binding affinity and kinetics using surface plasmon resonance biosensors.

@article{Schuck1997ReliableDO,
  title={Reliable determination of binding affinity and kinetics using surface plasmon resonance biosensors.},
  author={Peter Schuck},
  journal={Current opinion in biotechnology},
  year={1997},
  volume={8 4},
  pages={498-502}
}
  • Peter Schuck
  • Published 1997 in Current opinion in biotechnology
Progress has been made in the identification of experimental and analytical procedures that allow for a more reliable determination of equilibrium and kinetic constants. Possible origins of the frequently observed deviations of the measured binding progress from that expected for chemical binding of pseudo-first order, and appropriate experimental controls have been proposed. Improved analytical approaches include the application of global analysis and analytical corrections for the influence… CONTINUE READING

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