Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes.

@article{Xia2001ReliableAG,
  title={Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes.},
  author={Zhenfang Xia and Yong Liu},
  journal={Biophysical journal},
  year={2001},
  volume={81 4},
  pages={2395-402}
}
Green fluorescence protein (GFP)-based fluorescence resonance energy transfer (FRET) is increasingly used in investigation of inter- and intramolecular interactions in living cells. In this report, we present a modified method for FRET quantification in cultured cells using conventional fluorescence microscopy. To reliably measure FRET, three positive control constructs in which a cyan fluorescence protein and a yellow fluorescence protein were linked by peptides of 15, 24, or 37 amino acid… CONTINUE READING
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