Reliable and fast allele-specific extension of 3'-LNA modified oligonucleotides covalently immobilized on a plastic base, combined with biotin-dUTP mediated optical detection.

@article{Michikawa2006ReliableAF,
  title={Reliable and fast allele-specific extension of 3'-LNA modified oligonucleotides covalently immobilized on a plastic base, combined with biotin-dUTP mediated optical detection.},
  author={Yuichi Michikawa and Kentaro Fujimoto and Kenji Kinoshita and Seiko Kawai and Keisuke Sugahara and Tomo Suga and Y. Otsuka and Kazuhiko Fujiwara and Mayumi Iwakawa and Takashi Imai},
  journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry},
  year={2006},
  volume={22 12},
  pages={
          1537-45
        }
}
In the present work, a convenient microarray SNP typing system has been developed using a plastic base that covalently immobilizes amino-modified oligonucleotides. Reliable SNP allele discrimination was achieved by using allelic specificity-enhanced enzymatic extension of immobilized oligonucleotide primer, with a locked nucleic acid (LNA) modification at the SNP-discriminating 3'-end nucleotide. Incorporation of multiple biotin-dUTP molecules during primer extension, followed by binding of… CONTINUE READING

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