Reliability of polymerase chain reaction for detection of hepatitis C virus

@article{Zaaijer1993ReliabilityOP,
  title={Reliability of polymerase chain reaction for detection of hepatitis C virus},
  author={Hans L Zaaijer and H. T. M. Cuypers and Hendrik W Reesink and I N Winkel and Guido G H Gerken},
  journal={The Lancet},
  year={1993},
  volume={341},
  pages={722-724}
}
The polymerase chain reaction (PCR) is used to detect hepatitis C virus (HCV) RNA, and the results of this assay may have a bearing on management of patients. We tested 31 laboratories for performance of HCV PCR with a coded panel that comprised 4 HCV-positive plasma samples, 6 HCV-negative samples, and two dilution series of HCV-positive plasma. 15 (48%) laboratories had faultless results with both dilution series, and 16 (52%) laboratories reported erroneous results with one or both series… CONTINUE READING

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