Reliability of polymerase chain reaction (PCR) analysis of single cells for preimplantation genetic diagnosis

@article{Strom1994ReliabilityOP,
  title={Reliability of polymerase chain reaction (PCR) analysis of single cells for preimplantation genetic diagnosis},
  author={Charles M. Strom and Svetlana Rechitsky and George Wolf and Yury Verlinsky},
  journal={Journal of Assisted Reproduction and Genetics},
  year={1994},
  volume={11},
  pages={55-62}
}
We investigated the reliability of polymerase chain reaction (PCR) genotype analyses performed on single cells for the purposes of preimplantation genetic analysis. We performed blind analysis of 130 single skin fibroblasts heterozygous for the Δ-F508 mutation in the cystic fibrosis transmembrane regulator (CFTR) gene and 73 single skin fibroblasts from an individual heterozygous for the Xbapolymoporphic site of the Factor VIII gene. Amplification was successful for 116 cells and 52 cells… CONTINUE READING

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