Reliability of EMA binding test in the diagnosis of hereditary spherocytosis in Italian patients.

  title={Reliability of EMA binding test in the diagnosis of hereditary spherocytosis in Italian patients.},
  author={Elena D'Alcamo and Veronica Agrigento and Serena Sclafani and A. Z. P. Vitrano and Liana Cuccia and Aurelio Maggio and Silverio Perrotta and Marcello Capra and Paolo Rigano},
  journal={Acta haematologica},
  volume={125 3},
The eosin-5 -maleimide (EMA) binding test has been described for the diagnosis of HS [9–14] . This method is based on the degree of fluorescence of red blood cells after incubation with EMA dye, which reacts covalently with Lys 430 on the first extracellular loop of band 3 protein. It also interacts with Rh protein, Rh glycoprotein and CD 47 [15] . The EMA binding test has been shown to be an effective tool for diagnosis of HS in patients because it overcomes several difficulties concerning… CONTINUE READING
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