Relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound FAD.

Abstract

The flavoprotein cholesterol oxidase (CO) from Brevibacterium sterolicum is a monomeric flavoenzyme containing one molecule of FAD cofactor covalently linked to His69. The elimination of the covalent link following the His69Ala substitution was demonstrated to result in a significant decrease in activity, in the midpoint redox potential of the flavin, and… (More)
DOI: 10.1110/ps.073137708

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