Relevance of O-acetyl and phosphoglycerol groups for the antigenicity of Streptococcus pneumoniae serotype 18C capsular polysaccharide.

@article{Chang2012RelevanceOO,
  title={Relevance of O-acetyl and phosphoglycerol groups for the antigenicity of Streptococcus pneumoniae serotype 18C capsular polysaccharide.},
  author={Janoi Chang and Yohanna Serrano and Raine Garrido and Laura Rodriguez and Jessy Pedroso and Félix Cardoso and Yury Vald{\'e}s and Dagmar Garc{\'i}a and Violeta Fern{\'a}ndez-Santana and vicente Verez-Bencomo},
  journal={Vaccine},
  year={2012},
  volume={30 49},
  pages={
          7090-6
        }
}
Role of O-Acetylation in the Immunogenicity of Bacterial Polysaccharide Vaccines
TLDR
The structures of the bacterial capsular polysaccharide (CPS) antigens, extracted and purified from microbial cultures and obtained with very high purity, show that many of them are decorated by O-acetyl groups.
Procedure for the conjugation of the Streptococcus pneumoniae serotype 6B capsular polysaccharide to the tetanus toxoid
TLDR
The procedure described supports obtaining PS6B-TT conjugates reproducibly in the 30- 100 kDa and 10-30 kDa molecular size ranges and with 8-18 % oxidized repeat units, which are immunogenic.
Activación del polisacárido capsular de Streptococcus pneumoniae serotipo 23F para la obtención de vacunas conjugadas
TLDR
The procedure allows immunogenic conjugate from capsular polysaccharide serotype 23F of Streptococcus pneumoniae in rabbit models and the glycerol-phosphate substituent was found as important motif to preserve the antigenicity.
Non-stoichiometric O-acetylation of Shigella flexneri 2a O-specific polysaccharide: synthesis and antigenicity.
TLDR
The influence of the polysaccharide non-stoichiometric di-O-acetylation on antigenicity is addressed for the first time and mIgG F22-4 exemplifies a remarkable tight binding antibody against diversely O- acetylated and non-O’acetylated fragments of a neutral poly Saccharide of medical importance.
Impact of O-Acetylation on S. flexneri 1b and 2a O-Antigen Immunogenicity in Mice
TLDR
This work contributes to the optimization of vaccine design against Shigella, providing indication about the ability of shared epitopes to elicit broad protection against S. flexneri serotypes and supporting the identification of critical quality attributes of OAg-based vaccines.
An Overview of Structural Features of Antibacterial Glycoconjugate Vaccines That Influence Their Immunogenicity.
TLDR
The nature of the carrier protein, spacer and conjugation pattern used to develop the glycoconjugate vaccine also defines its overall spatial orientation which affects its avidity and selectivity of interaction with the desired target(s).
Bacterial Polysaccharide Vaccines: Analytical Perspectives
TLDR
This chapter presents background on the physical chemical nature of bacterial polysaccharides, their Critical Quality Attributes (CQA’s), and the analytical methodologies applied to their structural and physical characterization as well as to measure their CQA's.
In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.
TLDR
Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
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References

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Antibody Responses to Capsular Polysaccharide Backbone and O-Acetate Side Groups of Streptococcus pneumoniae Type 9V in Humans and Rhesus Macaques
TLDR
A study was undertaken to compare the relative contributions of O-acetate and Ps backbone epitopes in the immune response to S. pneumoniae 9V type-specific Ps and concluded that the O- acetate side groups are not essential to the ability of the 9V Ps to induce functional antibody responses.
Protective Meningococcal Capsular Polysaccharide Epitopes and the Role of O Acetylation
TLDR
There may be a generalized role for the O-acetyl group to provide an epitope of misdirected immunogenicity for meningococcal PS capsules, enabling escape from immune surveillance.
Streptococcus pneumoniae Type 14 Polysaccharide-Conjugate Vaccines: Length Stabilization of Opsonophagocytic Conformational Polysaccharide Epitopes
TLDR
A simple and convenient method was developed for the preparation of Streptococcus pneumoniae type 14 polysaccharide (Pn14PS)-tetanus toxoid (TT) conjugate vaccines, using terminally linked Pn 14PS fragments of different lengths, and the conformational epitope was found to be essential for the induction of antibodies with high opsonophagocytic activity.
Effect of O Acetylation of Neisseria meningitidis Serogroup A Capsular Polysaccharide on Development of Functional Immune Responses
TLDR
The dramatic reduction in immunogenicity associated with removal of O-acetyl groups indicates that O acetylation is essential to the immunogenic epitopes of serogroup A PS.
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TLDR
The dOA form of the GCMP vaccine may provide better protection against group C meningococcal disease than the OA form by eliciting a greater proportion of functional antibodies that are directly aimed at the protective epitope.
Structure of the specific capsular polysaccharide of Streptococcus pneumoniae type 23F (American type 23).
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  • Chemistry
    Biochemistry and cell biology = Biochimie et biologie cellulaire
  • 1988
TLDR
By composition analysis, optical rotation, partial hydrolysis, periodate oxidation, methylation, and high-resolution 1H and 13C nuclear magnetic resonance studies, the elucidated unambiguous structure was in agreement with an earlier proposal but is at variance with structures proposed later by other authors.
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