Relevance of Antibody Validation for Flow Cytometry

  title={Relevance of Antibody Validation for Flow Cytometry},
  author={Tomas Kalina and Kelly Lundsten and Pablo Engel},
  journal={Cytometry Part A},
Antibody reagents are the key components of multiparametric flow cytometry analysis. Their quality performance is an absolute requirement for reproducible flow cytometry experiments. While there is an enormous body of antibody reagents available, there is still a lack of consensus about which criteria should be evaluated to select antibody reagents with the proper performance, how to validate antibody reagents for flow cytometry, and how to interpret the validation results. The achievements of… 
Lot-to-lot reproducibility, stability and life cycle management of antibody reagents for flow cytometry.
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Reproducibility of Flow Cytometry Through Standardization: Opportunities and Challenges
  • T. Kalina
  • Biology
    Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • 2019
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This validated pipeline augments the laboratory's ability to provide rapid high‐throughput immune profiling for patients participating in cancer immunotherapy clinical trials and details the successful incorporation of an automated gating strategy with flowDensity into the analysis workflow.
EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols
The 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database.
Antibodypedia, a Portal for Sharing Antibody and Antigen Validation Data*S
A new publicly available portal called Antibodypedia is described, which has been developed to allow sharing of information regarding validation of antibodies in which providers can submit their own validation results and reliability scores.
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The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays, and the data processing approaches used to interpret the primary output data.
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A standardized immune phenotyping and automated data analysis platform for multicenter biomarker studies.
A platform that standardizes a set of flow cytometry panels across multiple centers, with high reproducibility in blood or PBMCs from either healthy subjects or patients 100 days after hematopoietic stem cell transplantation is reported.
The anatomy of single cell mass cytometry data.
This primer introduces the idiosyncrasies of mass cytometry data with a focus on the technical properties of how data generated with the CyTOF® system, and the characteristics of protein expression in the cells of the hematopoietic continuum, specifically targeted toward data scientists.
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