Release of the variant surface glycoprotein during differentiation of bloodstream to procyclic forms of Trypanosoma brucei.

@article{Buelow1989ReleaseOT,
  title={Release of the variant surface glycoprotein during differentiation of bloodstream to procyclic forms of Trypanosoma brucei.},
  author={Roger Buelow and C Nonnengaesser and Peter Overath},
  journal={Molecular and biochemical parasitology},
  year={1989},
  volume={32 1},
  pages={85-92}
}
Investigations on the turnover of the membrane-form variant surface glycoprotein (mfVSG) of Trypanosoma brucei during cultivation in vitro of the monomorphic variant clones MIT at 1.2 and MIT at 1.4 showed that bloodstream forms slowly released the surface coat into the medium (time required to decline to half the initial amount, t50% = 32 +/- 3 h). VSG appeared in the medium in its soluble form (sVSG) which lacked the dimyristoylglycerol membrane anchor as judged by electrophoretic mobility… CONTINUE READING
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