Release of leukotriene C4 from human polymorphonuclear leucocytes as determined by radioimmunoassay.

Abstract

Rabbits were immunized with a conjugate of leukotriene (LT) C4 and bovine serum albumin prepared by coupling the single free amino group of the hapten to the protein using gluteraldehyde. Binding of [3H]LTC4 to the antibodies obtained is inhibited by 50% with 1.5 ng LTC4. The relative cross-reaction of LTD4 is 16% and of LTC4-methyl ester 3.6%. The validity of the radioimmunoassay was demonstrated by comparison with bioassay using the isolated guinea pig ileum. Using the radioimmunoassay it could be shown that endogenous LTC4 is released in a dose-dependent manner by human polymorphonuclear leucocytes stimulated with the divalent cation ionophore A23187.

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@article{Aehringhaus1982ReleaseOL, title={Release of leukotriene C4 from human polymorphonuclear leucocytes as determined by radioimmunoassay.}, author={Ute Aehringhaus and R. H. W{\"{o}lbling and Wolfgang Koenig and Carlo Patrono and Brigitta M. Peskar and Bernhard A. Peskar}, journal={FEBS letters}, year={1982}, volume={146 1}, pages={111-4} }