Release of cytochrome c and activation of pro-caspase-9 following lysosomal photodamage involves bid cleavage

@article{Reiners2002ReleaseOC,
  title={Release of cytochrome c and activation of pro-caspase-9 following lysosomal photodamage involves bid cleavage},
  author={John J. Reiners and J A Caruso and Patricia A Mathieu and Bhadrani Chelladurai and X Yin and David Kessel},
  journal={Cell Death and Differentiation},
  year={2002},
  volume={9},
  pages={934-944}
}
Photodynamic therapy (PDT) protocols employing lysosomal sensitizers induce apoptosis via a mechanism that causes cytochrome c release prior to loss of mitochondrial membrane potential (ΔΨm). The current study was designed to determine how lysosomal photodamage initiates mitochondrial-mediated apoptosis in murine hepatoma 1c1c7 cells. Fluorescence microscopy demonstrated that the photosensitizer N-aspartyl chlorin e6 (NPe6) localized to the lysosomes. Irradiation of cultures preloaded with NPe6… CONTINUE READING