Release of active cytokinin by a beta-glucosidase localized to the maize root meristem.

@article{Brzobohat1993ReleaseOA,
  title={Release of active cytokinin by a beta-glucosidase localized to the maize root meristem.},
  author={Br̆etislav Brzobohat{\'y} and Ian Moore and Peter Kristoffersen and L{\'a}szl{\'o} Bak{\'o} and Narciso Campos and J. L. Schell and Klaus Palme},
  journal={Science},
  year={1993},
  volume={262 5136},
  pages={1051-4}
}
A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60.1) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3-glucoside, releasing active cytokinin. Tobacco protoplasts that transiently expressed Zm-p60.1 could use the inactive cytokinin glucosides to initiate cell division. The ability of protoplasts to sustain growth in response to cytokinin glucosides persisted indefinitely after the likely disappearance of the expression vector. In the… CONTINUE READING
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