Release of N2,3-ethenoguanine from chloroacetaldehyde-treated DNA by Escherichia coli 3-methyladenine DNA glycosylase II.

@article{Matijaevi1992ReleaseON,
  title={Release of N2,3-ethenoguanine from chloroacetaldehyde-treated DNA by Escherichia coli 3-methyladenine DNA glycosylase II.},
  author={Zdenka Matija{\vs}evi{\'c} and Mutsuo Sekiguchi and David B. Ludlum},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 19},
  pages={9331-4}
}
The human carcinogen vinyl chloride is metabolized in the liver to reactive intermediates which form N2,3-ethenoguanine in DNA. N2,3-Ethenoguanine is known to cause G----A transitions during DNA replication in Escherichia coli, and its formation may be a carcinogenic event in higher organisms. To investigate the repair of N2,3-ethenoguanine, we have prepared an N2,3-etheno[14C]guanine-containing DNA substrate by nick-translating DNA with [14C]dGTP and modifying the product with… CONTINUE READING