Release of ATP by a human retinal pigment epithelial cell line: potential for autocrine stimulation through subretinal space

@article{Mitchell2001ReleaseOA,
  title={Release of ATP by a human retinal pigment epithelial cell line: potential for autocrine stimulation through subretinal space},
  author={C. Mitchell},
  journal={The Journal of Physiology},
  year={2001},
  volume={534}
}
  • C. Mitchell
  • Published 2001
  • Biology, Medicine
  • The Journal of Physiology
1 Stimulation of purinergic receptors on retinal pigment epithelial (RPE) cells can increase the rate of fluid transport or decrease phagocytosis. This study aims to: determine whether the purine ATP can be released from RPE cells, begin probing the mechanism of any release and test whether cells degrade ATP extracellularly. 2 ATP release was monitored from cultured human ARPE‐19 cells with the luciferin‐luciferase assay. Biphasic release of ATP was triggered by basic fibroblast growth factor… Expand
Degradation of extracellular ATP by the retinal pigment epithelium.
TLDR
The results suggest that extracellular degradation of ATP in subretinal space can result in the production of ADP, which can stimulate P2Y receptors and augment Ca(2+) signaling in the RPE. Expand
Potentiation of ATP-induced Ca2+ mobilisation in human retinal pigment epithelial cells.
Interaction of signalling pathways directs the functional output of many cells. This study investigated the consequences of activating adenosine and adrenergic receptors on ATP-induced Ca2+ responsesExpand
PGE(2), Ca(2+), and cAMP mediate ATP activation of Cl(-) channels in pigmented ciliary epithelial cells.
TLDR
It is concluded that nucleotides can act at >1 P2Y receptor to trigger a sequential cascade involving Ca(2+), PGE(2), and cAMP, which could lead to net aqueous humor formation and intraocular pressure. Expand
Glutamate acts at NMDA receptors on fresh bovine and on cultured human retinal pigment epithelial cells to trigger release of ATP
TLDR
It is suggested that glutamate stimulates NMDA receptors on the apical membrane of RPE cells to release ATP, which can amplify the glutaminergic signal by increasing Ca2+ inside R PE cells, and might activate Ca2-‐dependent conductances. Expand
Release of ATP from retinal pigment epithelial cells involves both CFTR and vesicular transport.
TLDR
Results show an intimate involvement of CFTR in ATP release from RPE cells which can autostimulate receptors on the apical membrane to modify Ca(2+) signaling. Expand
Distinct P2Y receptor subtypes regulate calcium signaling in human retinal pigment epithelial cells.
TLDR
These data define the expression profile of P2y receptors in human RPE and show that different P2Y subtypes control distinct calcium responses in these cells. Expand
Final Accepted Version Degradation of Extracellular ATP by the Retinal Pigment Epithelium
Stimulation of ATP or adenosine receptors causes important physiologic changes in retinal pigment epithelial (RPE) cells that may influence their relationship to the adjacent photoreceptors. WhileExpand
Stimulation of the P 2 Y 1 Receptor UpRegulates Nucleoside-Triphosphate Diphosphohydrolase-1 in Human Retinal Pigment Epithelial Cells
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Stimulation of the P2Y1 Receptor Up-Regulates Nucleoside-Triphosphate Diphosphohydrolase-1 in Human Retinal Pigment Epithelial Cells
TLDR
Extended exposure to extracellular ATPγS increased NTPDase1 message and protein levels and increased ecto-ATPase activity, which reflects a feedback circuit, mediated at least in part by the P2Y1 receptor, to regulate levels of extacellular purines in subretinal space. Expand
Extracellular ATP Is an Autocrine/Paracrine Regulator of Hypoxia-induced Adventitial Fibroblast Growth
TLDR
It is concluded that hypoxia induces ATP release from endothelial cells and fibro Blasts and that the activation of P2 purinoceptors is involved in the regulation of DNA synthesis by fibroblasts under hypoxic conditions. Expand
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TLDR
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