Release of [14C]adenosine derivatives from superfused synaptosome preparations. Effects of depolarizing agents and metabolic inhibitors.

@article{Daval1980ReleaseO,
  title={Release of [14C]adenosine derivatives from superfused synaptosome preparations. Effects of depolarizing agents and metabolic inhibitors.},
  author={Jean Luc Daval and Claude E Barberis and Jacques Gayet},
  journal={Brain research},
  year={1980},
  volume={181 1},
  pages={
          161-74
        }
}
Adenine 5'-mononucleotides of synaptosome beds from guinea pig neocortex were labelled by incubation with [8-(14)C]adenosine, and excess adenosine was then removed with precursor-free medium. During continuous superfusion, labelled adenosine derivatives were released at a rate of about 0.5% of the synaptosome 14C/min and this rate was increased 2.5-fold by depolarization with 33 mM K+. Some depolarizing agents and metabolic inhibitors were examined for action on the release of [14C)-adenosine… CONTINUE READING